The canine HMGA1.
Conversely, HMGA1 overexpression is not associated to any rearrangement or amplification of the HMGA1 locus.
HMGA1 proteins in human atherosclerotic plaques.
Hmga1 is required for normal sperm development.
Regulation of microRNA expression by HMGA1 proteins.
Characterization of the Stoichiometry of HMGA1/DNA Complexes.
In this paper we report that this destabilization of HMGA1 mRNA is triggered by enhanced expression of RNA from an HMGA1 pseudogene, HMGA1-p.
HMGA1 antisense construct transfected into P+ cells blocked HMGA1 protein expression and inhibited TPA-induced transformation indicating that HMGA1 is required for transformation.
Sequence and analysis of the murine Hmgiy (Hmga1) gene locus.
Molecular mechanism of HMGA1 deregulation in human neuroblastoma.
Importantly, this recruitment of hSWI/SNF required HMGA1 proteins.
HMGA1 and HMGA2 protein expression in mouse spermatogenesis.
Thyroid cell transformation requires the expression of the HMGA1 proteins.
The canine HMGA1 gene maps to CFA 23.
Dynamic mitochondrial localization of nuclear transcription factor HMGA1.
Conversely, HMGA1 proteins were not detected in normal brain.
The HMGA1 proteins have a critical role in the process of carcinogenesis.
HMGA1 mediates the activation of the CRYAB promoter by BRG1.
We have found 11 proteins that associate with HMGA1.
HMGA1 protein is a novel target of the ATM kinase.
HMGA1 is a molecular determinant of chemoresistance to gemcitabine in pancreatic adenocarcinoma.
HMGA1 protein expression in familial breast carcinoma patients.
HMGA1 and HMGA2 rearrangements in mass-forming endometriosis.
HMGA1 expression was not associated with survival in pancreatic head cancer.
These findings suggest a pivotal role for HMGA1 in cancer.
From bending DNA to diabetes: the curious case of HMGA1.
HMGA1-pseudogene overexpression contributes to cancer progression.
Expression of HMGA1 and ezrin in laryngeal squamous cell carcinoma.
However, regulation of HMGA1 expression is not well understood.
Repression of HMGA1 by miR-296 is direct and sequence specific.
6p21 rearrangements in uterine leiomyomas targeting HMGA1.
Expression and role of HMGA1 in renal cell carcinoma.
HMGA1 rearrangements were present in 2 additional cases.
Molecular basis for the inhibition of HMGA1 proteins by distamycin A.
Crystal structure of a complex of DNA with one AT-hook of HMGA1.
The response of HMGA1 to changes in oxygen availability is evolutionarily conserved.
Evidence was only found for the existence of the "a" isoform of HMGA1 in fish.
HMGA1, a novel locus for type 2 diabetes mellitus.
The frequency of HMGA1 gene variants among cases and controls.
To determine the role of the HMGA1 gene in thyroid cell transformation, we blocked HMGA1 protein synthesis by an antisense methodology.
In fact, we found that Hmga1-/- ES cells overexpress GATA-1 and that HMGA1 proteins directly control GATA-1 transcription.
Re-expression of the Hmga1 gene in Hmga1 null ES cells restores the wild-type phenotype.
Therefore, suppression of HMGA1 protein synthesis by an HMGA1 antisense approach seems to be a feasible treatment strategy in pancreatic carcinomas.
In this study, to define HMGA1 function in vivo, we examined the consequences of disrupting the Hmga1 gene in mice.
Increased nuclear expression of HMGA1 and 2 correlated with poor survival (for adenocarcinomas, HMGA1, p=0.006; HMGA2, p=0.05).
Although HMGA1 proteins are overexpressed in most types of cancer, signaling circuits regulated by HMGA1 are not clarified in detail.
Additionally, stable overexpression of HMGA1 in MiaPaCa2 cells (characterized by low levels of inherent HMGA1 expression) was achieved.
"Best friends" sharing the HMGA1 gene: comparison of the human and canine HMGA1 to orthologous other species.
We demonstrate that the HMGA1 target sequences and HMGA1 proteins are required for the maximal activation of the CRYAB promoter by BRG1.
To determine how HMGA1 leads to neoplastic transformation, we looked for genes regulated by HMGA1 using gene expression profile analysis.
Interaction of adriamycin with a promoter region of hmga1 and its inhibitory effect on HMGA1 expression in A431 human squamous carcinoma cell line.
HMGA1 proteins have been recently found overexpressed in human leukemias, but to our knowledge this is the first reported duplication of HMGA1.
The overexpressed HMGA1 was found to form complexes with cellular RB, suggesting that downregulation of HMGA1 is required for G0 arrest.
Our present findings indicate that overexpression of HMGA1 disturbs RB-mediated cell arrest, suggesting a negative control of RB by HMGA1.
HMGA1 siRNA could remarkably reduce the expression of HMGA1 gene in ovarian cell and also inhabit the ovarian cell growth.
HMGA1 cDNA was stably transfected into MiaPaCa2 human pancreatic adenocarcinoma cells (which have low baseline expression levels of HMGA1).
HMGA1 silencing reduced igf-ir promoter activity whereas forced HMGA1 expression increased it.
Conversely, forced HMGA1 overexpression in cells with low endogenous HMGA1 levels was associated with IGF-IR upregulation.
These results suggested that HMGA1 is a positive regulator of miR-222, and HMGA1 overexpression might contribute to dysregulation of Akt signaling in NSCLC.
To determine how HMGA1 functions in metastatic colon cancer, we manipulated HMGA1 expression in transgenic mice and colon cancer cells.
Because HMGA1 chromatin remodeling proteins regulate transcription, HMGA1 is thought to drive malignant transformation by modulating expression of specific genes.
Furthermore, we identified cdc25A as a target of HMGA1 and showed that physical interaction between HMGA1 and the cdc25A promoter is required for transcriptional upregulation.
HMGA1 was found to interact with and activate the GLUT3 promoter and CAV1 increased the HMGA1 activity by enhancing its nuclear localization.
shRNA knockdown of HMGA1 in HeLa cells resulting in a decrease of HMGA1 showed a significant decrease of Vpr mRNA.
Interaction of doxorubicin with a regulatory element of hmga1 and its in vitro anti-cancer activity associated with decreased HMGA1 expression.
TGF-beta1 induces HMGA1 expression in human breast cancer cells: implications of the involvement of HMGA1 in TGF-beta signaling.
Lastly, the expression of the HMGA1 pseudogenes was significantly correlated with HMGA1 protein levels thereby implicating HMGA1P overexpression in cancer progression.
Patients with higher HMGA1 expression had a significantly shorter overall survival time than did patients with low HMGA1 expression.
New data show HMGA1 directly interacts with Ets-1 in addition to PU.1. In vitro HMGA1/Ets-1 interaction facilitates Ets-1/mu enhancer binding in the absence of an HMGA1.Ets-1.DNA complex.
Finally for a couple of analyzed HMGA1-regulated genes, electrophoretic mobility shift assay and chromatin immunoprecipitation revealed a direct binding of HMGA1 proteins to their promoters, ...
Using Hmga1-green fluorescent protein (GFP) knock-in mice that endogenously express an Hmga1-GFP fusion protein, we examined Hmga1 expression in undifferentiated and differentiated populations of ...
The data indicated that the distribution of complexes with different HMGA1 to DNA stoichiometries depended on the molar ratio of HMGA1 to DNA in solution, which could have significant biological ...
To investigate the influence of down-regulating HMGA1 on the tumor and the mechanism underlying antitumor of HMGA1, we transfected the HMGA1 shRNA vector into the osteogenic sarcoma MG-63 cell and ...
We further showed that HMGA1 silencing reduced miR-222 transcriptional activity, whereas forced HMGA1 expression increased it, indicating that miR-222 is directly regulated by HMGA1.
We found that F-actin recognized phospho-form of PKCepsilon and chaperoned it to the nuclear region where it interact with HMGA1 and Sp1, the transcription regulator of IR and HMGA1 gene ...
This could suggest that the binding of HMGA1 to DNA was not required for its stimulatory effect.
More recently, similar alterations of the HMGA1 gene have been described.
HMGA1 enhances the transcriptional activity and binding of the estrogen receptor to its responsive element.
In this study, the HMGA1 expression of human atherosclerotic plaque samples was examined.
IFN-gamma gene expression is controlled by the architectural transcription factor HMGA1.
The aim of this study was to determine the role of HMGA1 and HMGA2 throughout mouse spermatogenesis.
These three isoforms are encoded by the HMGA1 gene through alternative splicing.
HMGA1 protein over-expression is a frequent feature of epithelial ovarian carcinomas.
HMGA1 co-activates transcription in B cells through indirect association with DNA.
Furthermore, antisense HMGA1 substantially decreases mu enhancer activity in B cells.
We have found a strong variation in HMGA1 expression between the tumours.
HMGA1 and HMGA2 have also been found to stimulate retroviral integration in vitro.